Fig. 3

TP53 mutation screening and conventional cytogenetics. a SNP in exon 4 (forward and reverse) and missense mutations in exon 5 (forward and reverse). b Representative karyotype of one ZJU-0327 cells at the P35: 81. Red arrows point to der (2) t (2;9) (p23;q21), +i(3) (q10), +del (3) (q13.1)×2, +4, +5, +7, +8, +add (8) (p23), +der (8) t (8;?) (p12;?), −9×3, −10, +11×2, +12, +add (14) (p13), +15, −16, −17, +der(19) t (19;?) (q13.3;?), +der(21) t (21;?) (q22;?), −22×2 [CP3]. c Representative karyotype of ZJU-0725 cells at the P25: 78. Red arrows point to der(X)t(X;?)(q21;?),i(X)(q10),−2,add(2)(p25),der(2)t(2;?)(p11.2;?),del(3)(q12)×2,+i(3)(q10)×2,del(5)(q11.2),−6,i(6)(p10),+der(8)t(8;?)(p12;?),−9×3,der(9)t(9;?)(p13;?),−10×2,−11,−12,−13×2,−14×2,−17×2,−18,−19×2,add(19)(p13.3),add(21)(q22)×2,−22×3,add(22)(q13),+mar1,+mar2,+mar3,+mar4,+mar5[cp10]. d Representative karyotype of ZJU-1127 cells at the P23: 133 der(X)t(X;?)(p11.2;?)×3,i(X)(q10),+der(1)t(1;9)(p10;q10)×4,−2,add(2)(p25),+i(3)(q10)×8,−5,−6×2,+7×2,+der(8)t(8;?)(p12;?),+der(9)t(9;?)(p13;?)×5,der(10)t(10;?)(q22;?),+del(11)(p11.2)×2,+12,+13,−14×2,−15,−16×2,der(16)t(16;?)(q12;?)×2,−17×2,+der(18)t(18;?)(q11.2;?)×2,+add(19)(p13.3)×2,add(21)(p13),+add(21)(q22)×6,+add(22)(q13)×2,+mar1,+mar2,+mar3[1]