Table 1 Primer sequences for amplification of TP53 exons [13]
Target | Forward primer (5′→3′) | Reverse primer (5′→3′) | Product (bp) | Codon position |
|---|---|---|---|---|
Exon 1 | GTCGGCGAGAATCCTGACT | CCAACAATGCAACTCCTATGAT | 481 | 0 |
Exon 2 | TCAGACACTGGCATGGTGTT | GGGGACAGCATCAAATCATC | 498 | 1–25 |
Exon 3 | TCAGACACTGGCATGGTGTT | GGGGACAGCATCAAATCATC | 498 | 25–32 |
Exon 4 | GGGACTGACTTTCTGCTCTTGT | GCCAAAGGGTGAAGAGGAATC | 543 | 33–125 |
Exon 5 | GTTTCTTTGCTGCCGTCTTC | TTCCTTCCACTCGGATAAGATG | 387 | 126–187 |
Exon 6 | TACAAGCAGTCACAGCACATGA | GGTCAAATAAGCAGCAGGTTAAGA | 370 | 187–224 |
Exon 7 | GTGAAACCCCGTCTCTACTGAA | GAGGAGAAGCCACAGGTTAAGA | 588 | 225–261 |
Exon 8 | GGAGTAGATGGAGCCTGGTTTT | GTTGGGCAGTGCTAGGAAAG | 328 | 261–307 |
Exon 9 | GGTAAGCAAGCAGGACAAGAAG | TACAACCAGGAGCCATTGTCTT | 313 | 307–331 |
Exon 10 | CATGTTGCTTTTGTACCGTCAT | TGGATACACTGAGGCAAGAATG | 408 | 332–367 |
Exon 11 | AACATATTTGCATGGGGTGTG | CCAGTCTCCAGCCTTTGTTC | 1580 | 367–394 |