Fig. 2

TK1 co-localizes with the plasma membrane of lung, breast, and colon cell lines. NCI-H460, HT-29, and MD-MBA-231 cells were stained with FITC-conjugated antibodies and a cell membrane specific dye (Rhodamine). Unstained cells, cells stained with isotype antibody, and cells stained with anti-NFkB antibody (FITC) were used as controls to determine the viability of cells and the non-internalization of the antibodies to prevent false positives. FITC images show staining on cells treated with anti-TK1 antibody A74. The FITC images were merged with the Rhodamine images, which show the plasma membrane. The images show a clear co-localization of TK1 with the plasma membrane of these cells