Fig. 1

BC094916 overexpression in SP 2/0 cells. a Stable expression of BC094916 in SP 2/0 cells. BC094916- and EGFP-expressing lentiviral vector LV201 (BC094916) and only EGFP-expressing LV201 (vector) were transduced into SP 2/0 cells. Puromycin was used to select stable BC094916- and EGFP-expressing SP 2/0 cells. EGFP expression was shown. b BC094916 mRNA expression significantly increased in stable BC094916- and EGFP-expressing SP 2/0 cells. BC094916 mRNA expression was determined in the stable BC094916- and EGFP-expressing SP 2/0 cells (BC094916) and only EGFP-expressing SP 2/0 cells (vector) after cells were cultured for 48 h. c BC094916 protein expression significantly increased in BC094916-EGFP (fusion form of BC094916 carbon terminal and EGFP)-expressing SP 2/0 cells. BC094916-EGFP (BC094916)- or only EGFP (vector)-expressing lentiviral vector LV122 were transduced into SP 2/0 cells. At 72 h after transduction, cells were collected and anti-EGFP antibodies were used to probe EGFP or BC094916-EGFP fusion protein expression by western blot assay. a–c Data are representative of three independent experiments. b Two-tailed student’s t test. Error bars, SEM. ****p < 0.0001