Fig. 5

Transcription factor BC094916 suppressed the activation of Creb and Bcl2 promoter. a BC094916 located in the nuclear. 293T cells were transfected with BC094916-EGFP (BC094916)-, BC094916-C (the C-terminal of BC094916)-EGFP (BC094916-C) or only EGFP (vector)-expressing LV122, and cultured for 2 days. C ells were imaged and analyzed on a GE IN Cell Analyzer 2000. Representative images showed nuclear location of BC094916. b BC094916 suppressed the activation of Creb and Bcl2 promoter. BC094916 -expressing LV201 (BC094916) or empty vector LV 201 (Vector) and luciferase reporter vector pEZX-PG04.1/Creb1 promoter (− 1510 ~ + 173 bp) or pEZX-PG04.1/Bcl2 promoter (− 1323 ~ + 160 bp) were co-transduced into 293T (left panel) or SP 2/0 (middle and right panels) cells. Dual luciferase reporter gene expression was analyzed, and the results are shown as the ratio of firefly to Renilla luciferase activity. The data represent at least four independent experiments. Error bars, SEM. Two tailed Student’s t-test, *P < 0.05, **P < 0.01