Fig. 5

ZEB2 is a downstream target of miR-181b. a The putative binding sites between miR-181b and ZEB2 3′UTR. b Luciferase activity was determined in HEK293T cells co-transfected with miRNAs (mimics NC or miR-181b mimics) and reporter vector containing ZEB2 3′UTR segments (WT or MUT) miR-181b. c The mRNA expression of ZBE2 in SW1990 and PANC-1 cells transfected with mimic NC or miR-181b mimc was determined by qRT-PCR. d The mRNA expression of ZEB2 was up-regulated in pancreatic cancer tissues (n = 84) compared to normal adjacent pancreatic cancer tissues (n = 84). e The correlation between miR-181b expression and ZEB2 mRNA expression in pancreatic cancer tissues was analyzed by Spearman’s correlation test (r = − 0.2512, P = 0.0212). f The mRNA and g protein expression of ZEB2, E-cadherin, vimentin and N-cadherin in SW1990 cells co-transfected with siNC + inhibitor NC, siDLX6-AS1 + inhibitor NC or siDLX6-AS1 + miR-181b inhibitor. *P < 0.05 and ***P < 0.001