Fig. 4

RNAi was utilized to silence CLDN2 expression in osteoblast line. a Western blotting was utilized to examine the effects of silencing CLDN2 and the activation of the Ras/Raf/MEK/ERK signaling pathway in the osteoblast line. b The corresponding statistical analysis of the activation of the Ras/Raf/MEK/ERK signaling pathway. c The wound healing assay was utilized to detect the migration ability of the osteoblast line in vitro. d The transwell chamber method was utilized to detect the impact of CLDN2 silencing on the invasive ability of cells in vitro. e The corresponding statistical analysis of invaded cells. **P < 0.01 compared with the scramble group