Table 1 Sequences of primers and short RNA oligos
Gene | Forward primers | Reverse primers |
|---|---|---|
SKA3 | 5′-CAGATCCCTCTTCACCTACGA-3′ | 5′-TCAACGTTTAAAGGGGGACA-3′ |
β-Actin | 5′-GGCATCCTCACCCTGAAGTA-3′ | 5′-GGGGTGTTGAAGGTCTCAAA-3′ |
Over-SKA3 | 5′-GCTCTAGAGCCACCATGGACCCTATCCGGAGCTTCTGC-3′ | 5′-CGGAATTCTCACTTGTCATCGTCATCCTTGTAGTCGTTTTCTTTGTTGCTGACATCTCGGATG-3′ |
Sh-SKA3 | CCGGCATGGACAGAACATCCGAGATCTCGAGATCTCGGATGTTCTGTCCATGTTTTTTG | |