Table 2 Primer sequences for qRT-PCR
| Gene | Primer sequence |
|---|---|
| β-actin |
F: 5′- AGCGAGCATCCCCCAAAGTT -3′ R: 5′- GGGCACGAAGGCTCATCATT -3′ |
| LYN |
F: 5′- TGTGAGAGATCCAACGTCCA -3′ R: 5′- AAACTGCCCTTGGCCATGTA -3′ |
| MiR-218-5p |
F: 5′- CGAGTGCATTTGTGCTTGATCTA -3′ R: 5′- TAATGGTCGAACGCCTAACGTC -3′ |
| U6 |
F: 5′- CTCGCTTCGGCAGCACA -3′ R: 5′- AACGCTTCACGAATTTGCGT -3′ |
| si-LYN |
Sense: 5′- GCAUGGAGAAUGGUGGAAA -3′ Antisense: 5′- UUUCCACCAUUCUCCAUGC -3′ |
| si-LYN control |
Sense: 5′- GCAAGAGUAGGGUGUGAAA -3′ Antisense: 5′- UUUCACACCCUACUCUUGC -3′ |
| MiR-218-5p mimics | 5′- UUGUGCUUGAUCUAACCAUGU -3′ |
| MiR-218-5p mimics control | 5′- UCACAACCUCCUAGAAAGAGUAGA -3′ |
| MiR-218-5p inhibitor | 5′- AACACGAACUAGAUUGGUACA -3′ |
| MiR-218-5p inhibitor control | 5′- UUGUACUACACAAAAGUACUG -3′ |