Fig. 3

MiR-133b regulated EGFR expression in ESCC cells. a, b The level of miR-133b and mRNA level of EGFR in KYSE150 and ECa109 cells after transfection with miR-133b NC, miR-133b agomir, shEGFR, miR-133b antagomir and shEGFR were determined by RT-qPCR. c The protein level of EGFR in KYSE150 and ECa109 cells was detected by western blotting. d The gray-scale value of the bands were quantitatively analyzed. The experimental data were representative of three independent experiments. Results were expressed as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001