Fig. 2

miR-133b directly and essentially controlled FGFR1 expression in OS cells. a Bioinformatics predicted the potential binding site between miR-133b and the 3′-UTR of FGFR1. b Luciferase assay showed miR-133b directly inhibited FGFR1. The luciferase activities were measured at 48 h after co-transfecting MG-63 or U2OS cells the luciferase reporter driven by either WT or MUT FGFR1 3′-UTR and miR-133b mimics or control miRNA (miR-133b NC). c, d The relative expressions of miR-133b (c) and FGFR1 mRNA (d) in MG-63 (left panel) and U2OS cells (right panel) after transfected with miR-133b mimics vs. miR-133b NC, or shFGFR1 vs. shNC were measured by qRT-PCR. e, f The relative expression of FGFR1 protein in MG-63 (left panel) and U2OS cells (right panel) after transfected with miR-133b mimics vs. miR-133b NC, or shFGFR1 vs. shNC was measured by Western blotting. The representative images of Western blotting were shown in e and the quantification of the signals was presented as mean ± standard deviation from three independent experiments shown in f. *P < 0.05, **P < 0.01 and ***P < 0.001