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Fig. 2 | Cancer Cell International

Fig. 2

From: Overexpression of CD59 inhibits apoptosis of T-acute lymphoblastic leukemia via AKT/Notch1 signaling pathway

Fig. 2

The expression of wild-type CD59 is beneficial to the survival of Jurkat cells. a The cell vitality detected by CCK8 assay. b The dead cells detected by trypan blue staining. c Quantitative analysis of blue-stained cells. d Lytic cells detected by Dye release assay. e The apoptosis detected by flow cytometry. f Quantitative analysis of apoptosis. g The protein expression of Bax and Bcl-2 detected by western blot. h Quantitative analysis of mRNA expression of Bax and Bcl-2. i Quantitative analysis of protein expression of Bax and Bcl-2. j The content of IL-2 in culture medium detected by ELISA. All experiments were performed three times. *P < 0.05. n.s no significant

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Cancer Cell International

ISSN: 1475-2867

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