Fig. 3

PTEN was modulated by CASC2 and miR-21. PANC-1 cells were transfected with the CASC2-expressing vector (pEX-CASC2), CASC2 shRNA (shCASC2) and miR-21 mimics, miR-21 inhibitor or the corresponding negative controls for 48 h. Then, PTEN expression was detected by western blotting. a MiR-21 mimics downregulated PTEN. b CASC2 upregulated PTEN. c MiR-21 mimics abolished the effect of CASC2 on PTEN expression. ***P < 0.001