Fig. 2

Effects of RZM, Ros and/or Met on cellular proliferation. a–c The antiproliferative effects induced by RZM, the mixture of Ros and Met, Ros and Met in HepG2, SK-Hep1 and L02 cells were detected by MTS assay (*P < 0.05 and **P < 0.01 versus the control group). d Representative images of colony formation of differently treated HCC cells were shown. e Quantitative results of colony formation assays were displayed (*P < 0.05 and **P < 0.01 versus the control group). All values were expressed as the means mean ± SD from at least three independent experiments. f HepG2 cells were treated with 1 mM RZM or Met for 48 h, flow cytometric analysis was used to determine the effects of the two compounds on cellular apoptosis. The apoptotic rates were represented by the ratios of early and late apoptotic cells (P2-Q3% + P2-Q2%). g Quantitative results of flow cytometric analysis were displayed (*P < 0.05 versus the control group)