Fig. 3

Hsa_circ_0081143 served as a sponge of miR-646. a The estimated binding sites of hsa_circ_0081143 and miR-646. b TCGA database showed that miR-646 expression was significantly decreased in GC tissues. c Kaplan–Meier Plotter revealed that low miR-646 expression was associated with poor overall survival of GC patients. d Relative expression of miR-646 in 30 pairs of GC tissues by qRT-PCR. e Hsa_circ_0081143 expression was negatively correlated with miR-646 expression in GC tissues. f Downregulation of hsa_circ_0081143 suppressed the expression of miR-646 in GC cells. g Ectopic expression of miR-646 remarkably reduced the luciferase activity of wild-type but not mutant-type of circ_0081143. h RNA pull-down assay revealed that hsa_circ_0081143 was significantly enriched by biotin-miR-646 in GC cells. *P < 0.05