Fig. 4

CDK6 is a direct target for miR-646. a The estimated binding sites of CDK6 and miR-646. b TCGA database showed that CDK6 expression was significantly upregulated in GC tissues. c Kaplan–Meier Plotter revealed that high CDK6 expression was associated with poor overall survival of GC patients. d QRT-PCR showed CDK6 expression was significantly decreased in GC tissues. e IHC results showed that CDK6 protein expression was increased in GC tissues. f An inverse correlation was found between the expression level of miR-646 and CDK6. g MiR-646 mimics decreased the luciferase activity of wild-type 3′-UTR of CDK6. h, i Relative CDK6 mRNA and protein levels in GC cells treated with miR-646 mimics were determined by qRT-PCR and western blotting, respectively. *P < 0.05