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Fig. 9 | Cancer Cell International

Fig. 9

From: Role of microRNA-92a in metastasis of osteosarcoma cells in vivo and in vitro by inhibiting expression of TCF21 with the transmission of bone marrow derived mesenchymal stem cells

Fig. 9

TCF21 is suggested to be a direct target gene of miR-92a. a Detection of TCF21 mRNA levels in OS cells before and after co-culture with BMSCs by qRT-PCR; b detection of TCF21 protein expression in OS cells before and after co-culture with BMSCs by western blot analysis; c detection of TCF21 mRNA expression in OS cells of each group by qRT-PCR; d detection of TCF21 protein expression in OS cells by western blot analysis; e online prediction of targeting relationship between miR-92a and TCF21; f identification of targeting relationship between miR-92a and TCF21 by luciferase activity determination; Repetitions = 3; the data was analyzed by the t test or one-way ANOVA; After ANOVA analysis, the LSD-t was used for pairwise comparison; *P < 0.05 vs. the blank or the mimics NC group

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