Fig. 2
From: Low-dose zoledronate for the treatment of bone metastasis secondary to prostate cancer
Cell proliferation using alamarblue® assay (a) and Vybrant MTT® assay (b) of LAPC4 cells treated with vehicle (PBS1x) or zoledronate 1 µM, 3 µM, 10 µM and 100 µM for 7 days in 1% serum conditions. The histograms in (a) and (b) represent the ratio of drug-treated cells divided by vehicle-treated cells (PBS1x) in three independent experiments for all variables except for 100 µM that was done as a single experiment in triplicate. c Representative photos of Live/Dead assay carried out on LAPC4 following vehicle or zol treatment at different concentrations. Live cells are in green and dead cells are in red. d Percentage of viable cells [number of live cells/(number of live cells + number of dead cells) * 100] and e ratio of live cells or dead cells in vehicle or zol-treated conditions, Results are mean ± SD, p < 0.05. Scale bar 250 µm