Fig. 4

MiR-1180 contributed to ovarian cancer cell proliferation and glycolysis. a miR-1180 expression in BM-MSCs. BM-MSCs were transfected with miR-1180 inhibitors or the miRNA inhibitor controls for 48 h and the RNA was extracted for real time RT-PCR. b–d SKOV3 and COC1 cell proliferation was assayed by MTT method. SKOV3 and COC1 cells were treated with the conditioned medium (CM) from the BM-MSCs with miR-1180 down-regulation by the inhibitors transfection. Cell growth was measured at day 1, 2, 3, 4 and 5. e, f SKOV3 and COC1 cell survival ability was assayed by colony formation assay. SKOV3 and COC1 cells were treated with the conditioned medium (CM) from the BM-MSCs with miR-1180 down-regulation by the inhibitors transfection. The cells were cultured for 10 days and the colonies were counted. g, h SKOV3 and COC1 ovarian cancer cell ECAR was assayed. SKOV3 and COC1 cells were treated with the conditioned medium (CM) from the BM-MSCs with miR-1180 down-regulation by the inhibitors transfection for ECAR analysis. a: glucose; b: Oligomycin; c: 2-DG. i, j ATP production in SKOV3 and COC1 ovarian cancer cell was assayed. SKOV3 and COC1 cells were treated with the conditioned medium (CM) from the BM-MSCs with miR-1180 down-regulation by the inhibitors transfection for ATP production analysis. **p < 0.01; *p < 0.05