Fig. 1

Phosphorylated ITK expression in AITL patients and malignant T cell lymphoma cell lines. a Immunohistochemical staining analysis of the expression of phosphorylated ZAP70, ITK and PLCγ1 in paraffin-embedded tumor tissues from AITL patients and representative pictures. Each sample was graded independently by two pathologists. b, c Kaplan–Meier curve of PFS and OS according to the expression of phosphorylated ITK. d, e ITK and phosphorylated ITK expression level in malignant T cell lymphoma cell lines were analyzed using real-time PCR and Western blot. Jurkat, Hut-78 and H9 cells expressed total ITK and phosphorylated ITK as a band at 72 kDa. GAPDH is shown as a loading control (36 kDa). Data are expressed as Mean ± SD and representative of three independent experiments