Fig. 4

Autophagy inhibition partly abolish the oxaliplatin-resistance by co-culturing HCC cells with macrophages. a, b Western blot analysis of ATG5 conversion in SMMC-7721 and Huh-7 cells which transfected with Negative siRNA or ATG5-siRNA. c, d After transfected with Negative siRNA or ATG5-siRNA, the macrophages co-cultured SMMC-7721 and Huh-7 cells were exposed to 10 μM oxaliplatin for 12–48 h. The percentage of survived cells was determined with MTT assay and The percentage of apoptotic cells was determined with Annexin V staining assay. Data shown are mean (SD) from at least 3 independent experiments. Means were compared between two groups using unpaired, two-tailed Student’s t-test. * P < 0.05, ** P < 0.01