Fig. 2
From: MKP-4 suppresses hepatocarcinogenesis by targeting ERK1/2 pathway
MKP-4 regulates the phosphorylation of ERK1/2 in liver tumor cells. a The expressions of MKP-4 in LO2 and different liver tumor cells were detected by using western blot. b We used RNA interference to knockdown MKP-4 expression in HepG2 or SK-Hep1 cells and chose best interfering efficiency. The bar chart demonstrated the ratio of MKP-4 expression to GAPDH by densitometry. The data were mean ± SEM of three independent experiments (*P < 0.05). c The expressions of ERK1/2, p-ERK1/2 and the downstream target genes in different treated cells. The bar chart showed the ratio of these proteins to GAPDH by densitometry. The data were mean ± SEM of three independent experiments. (*P < 0.05)