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Fig. 5 | Cancer Cell International

Fig. 5

From: Melatonin enhances TNF-α-mediated cervical cancer HeLa cells death via suppressing CaMKII/Parkin/mitophagy axis

Fig. 5

Mitophagy was modulated by melatonin via the CaMKII-Parkin pathway. ad Western blotting was used to analyze the expression of CaMKII and Parkin in response to melatonin and/or TNF-α treatment. siRNA against Parkin was transfected into TNF-α-treated cells to inhibit Parkin-related mitophagy. eg Proteins were isolated from cells and the expression of p-CaMKII and Parkin was determined via western blotting. CaMKII specific agonist bradykinin (Brad) was added into melatonin-treated cells to re-activate the CaMKII pathway. H-J. Immunofluorescence assay for p-CaMKII and Parkin under melatonin and/or TNF-α treatment. CaMKII specific agonist bradykinin (Brad) was added into melatonin-treated cells to re-activate the CaMKII pathway. *P < 0.05 vs. ctrl group, #P < 0.05 vs. TNF-α group, @P < 0.05 vs. TNF-α + melatonin group

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