Fig. 9

Inactivated Akt signaling pathway inhibits in vivo tumorigenicity and tumor growth of OCSCs. a In vivo limiting dilution assay was used to detect in vivo stem cell percentage of ST6GALNAC1 in mice; b after transfection, the tumor size of different cells in nude mice was determined; c tumorigenic tumor weight in nude mice; d changes of tumor volume in nude mice; e β-catenin staining intensity in OCSCs detected by immunohistochemical staining (×200). The measurement data were expressed as mean ± standard deviation. The in vivo stem cell ratio of ST6GALNAC1 in mice and quantification of tumor volumes were analyzed by one-way ANOVA. The quantification of tumor weights was examined by repeated-measurement ANOVA (n = 6). *p < 0.05 vs. the empty vector group; the experiment was repeated 3 times