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Fig. 4 | Cancer Cell International

Fig. 4

From: PITX2 enhances progression of lung adenocarcinoma by transcriptionally regulating WNT3A and activating Wnt/β-catenin signaling pathway

Fig. 4

PITX2 regulated WNT3A transcription by directly binding to its promoter region. a The TF binding site of PITX2 was shown by Animal TF DataBase. b Predicted by hTFtarget website, PITX2 directly binds to the promoter region (1092–1101 bp) of WNT3A. c Ectopic expression of PITX2 increased wild type but not mutant WNT3A promoter luciferase activity (p < 0.001). d, e In ChIP assay, PITX2 antibody recruited WNT3A promoter DNA fragment. The IgG antibody and control primer were used as negative controls. f The expression of WNT3A were decreased by knockdown of PITX2 (p < 0.001). g In TCGA dataset, PITX2 had a positive correlation with WNT3A (R = 0.22, p < 0.001) (Fgiure4G) (*p < 0.05, **p < 0.01, ***p < 0.001)

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