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Table 1 Effect of DA-9401 on body, reproductive organ weight, serum hormone concentration, sperm count, motility, and fertility parameters in adriamycin treated male SD rats

From: Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague–Dawley rats

Parameter CTR DA 100 ADR ADR + DA 100 ADR + DA 200
Body and organ weight
 Body weight (beginning; gm) 229.00 ± 3.97 225.10 ± 3.41 235.91 ± 3.66 239.22 ± 5.37 232.00 ± 2.10
 Body weight (sacrifice; gm) 413.40 ± 9.12 409.20 ± 9.15 320.80 ± 7.94*# 369.88 ± 13.61*#+ 341.50 ± 7.98*
 Testis weight (gm) 2.03 ± 0.05 2.06 ± 0.02 0.81 ± 0.05*# 0.91 ± 0.03*# 1.14 ± 0.12*+
 Epididymis weight (gm) 0.72 ± 0.14 0.71 ± 0.01 0.48 ± 0.02*# 0.59 ± 0.02*#+ 0.56 ± 0.03*+
 Seminal vesicles weight (gm) 1.34 ± 0.04 1.45 ± 0.05 0.66 ± 0.06*# 1.03 ± 0.08*#+ 0.94 ± 0.07*+
 Prostate weight (gm) 0.97 ± 0.05 1.16 ± 0.07 0.67 ± 0.05*# 0.90 ± 0.04#+ 0.66 ± 0.04*$
 Penis weight (gm) 0.31 ± 0.01 0.31 ± 0.01 0.26 ± 0.01*# 0.30 ± 0.01+ 0.28 ± 0.01
Serum hormone levels
 Serum testosterone (ng/ml) 5.36 ± 0.96 5.53 ± 0.58 0.42 ± 0.07*# 1.62 ± 0.13*# 2.75 ± 0.51*+
 Serum LH (mIU/ml) 39.10 ± 4.07 46.17 ± 2.41 101.26 ± 8.67*# 84.33 ± 11.60*# 88.27 ± 10.62*
 Serum FSH (ng/ml) 4.07 ± 0.58 4.57 ± 0.88 14.30 ± 1.68*# 4.41 ± 1.85+ 4.41 ± 0.23+
Sperm count and motility
 Sperm count (106/ml)
  Vas deference 29.14 ± 0.99 26.64 ± 1.82 17.35 ± 0.89*# 22.42 ± 0.79*#+ 22.28 ± 0.28*+
  Epididymis 37.78 ± 1.28 34.78 ± 0.79 10.42 ± 2.01*# 16.85 ± 0.96*#+ 18.28 ± 1.64*+
 Sperm motility (%)
  Vas deference 65.72 ± 3.06 60.60 ± 4.67 31.50 ± 2.12*# 48.13 ± 2.72*+ 39.99 ± 1.85*
  Epididymis 36.78 ± 1.45 38.92 ± 2.19 15.33 ± 1.71*# 26.55 ± 1.65*#+ 22.57 ± 1.50*+
Fertility parameters
 Fertility rate (%) 100 100 50*# 80 100+
 Pups per female 12.80 ± 0.78 11.90 ± 0.56 5.60 ± 1.96*# 9.3 ± 1.78 10.50 ± 0.85+
  1. Results are expressed as mean ± SEM
  2. CTR, control; DA 100, DA-9401 100 mg/kg/day p.o.; ADR, adriamycin 2 mg/kg i.p. per week; ADR + DA 100, adriamycin 2 mg/kg i.p. per week + DA-9401 100 mg/kg/day p.o.; ADR + DA 200, adriamycin 2 mg/kg i.p. per week + DA-9401 200 mg/kg/day p.o. LH, luteinizing hormone; FSH, follicle stimulating hormone; p.o., per oral; i.p., intraperitoneally
  3. *P < 0.05 vs. CTR group, #P < 0.05 vs. DA 100 group, +P < 0.05 vs. ADR group, $P < 0.05 vs. ADR + DA 100 group (one-way ANOVA followed by the Tukey post hoc test; n = 10 for each group)