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Table 2 Effect of DA-9401 on biomarkers of oxidative stress and inflammatory in adriamycin treated male SD rats

From: Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague–Dawley rats

Parameter CTR DA 100 ADR ADR + DA 100 ADR + DA 200
MDA (μmol/mg protein) 6.83 ± 1.16 8.45 ± 0.70 14.75 ± 2.87* 7.99 ± 1.25+ 3.40 ± 0.35+
ROS/RNS (nanomole DCF/mg protein) 458.55 ± 17.71 397.75 ± 67.78 753.61 ± 30.71*# 300.91 ± 26.15+ 296.65 ± 59.45+
SOD level (units/mg protein) 6.29 ± 0.56 6.26 ± 0.32 2.76 ± 0.10*# 5.99 ± 0.46+ 4.80 ± 0.13+
GPx (nanomoles/min/mg protein) 44.29 ± 3.72 51.47 ± 3.42 18.20 ± 1.56*# 40.47 ± 5.08+ 50.09 ± 3.88+
Catalase (nanomoles/min/mg protein) 116.88 ± 6.51 122.83 ± 9.01 65.01 ± 2.48*# 132.21 ± 18.02+ 112.06 ± 6.90+
IL-6 (pg/mg protein) 1264.93 ± 32.16 1336.43 ± 129.61 3685.30 ± 497.63*# 1569.39 ± 370.72+ 1341.60 ± 109.25+
TNF-α (pg/mg protein) 1174.73 ± 55.29 1197.43 ± 152.88 3182.75 ± 232.87*# 1739.86 ± 182.09+ 1590.79 ± 107.5+
  1. Results are expressed as mean ± SEM
  2. CTR, control; DA 100, DA-9401 100 mg/kg/day p.o.; ADR, adriamycin 2 mg/kg i.p. per week; ADR + DA 100, adriamycin 2 mg/kg i.p. per week + DA-9401 100 mg/kg/day p.o.; ADR + DA 200, adriamycin 2 mg/kg i.p. per week + DA-9401 200 mg/kg/day p.o. MDA, malondialdehyde; ROS/RNS, reactive oxygen species/reactive nitrogen species; SOD, superoxide dismutase; GPx, glutathione peroxidase; IL-6, interleukin-6; TNF-α, tumor necrosis factor- α; p.o., per oral; i.p., intraperitoneally
  3. P < 0.05 vs. CTR group, #P < 0.05 vs. DA 100 group, +P < 0.05 vs. ADR group (one-way ANOVA followed by the Tukey post hoc test; n = 10 for each group)