Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: Silencing long non-coding RNA HOTAIR exerts anti-oncogenic effect on human acute myeloid leukemia via demethylation of HOXA5 by inhibiting Dnmt3b

Fig. 2

Silencing HOTAIR inhibits proliferation and promotes apoptosis of AML cells. a Protein expression of Bax, Bcl-2, MCP-1, cleaved-caspase3, p27 and cyclin G in HL-60 cells after interference with HOTAIR examined by western blot analysis. b Cell apoptosis of HL-60 cells after interference with HOTAIR examined by flow cytometry. c Cell cycle distribution of HL-60 cells after interference with HOTAIR detected by flow cytometry. d The proliferation of HL-60 cells after interference with HOTAIR examined by EdU assay (×200). *p < 0.05 vs. the sh-NC group. RT-qPCR reverse transcription quantitative polymerase chain reaction, HOTAIR Hox transcript antisense intergenic RNA, HOXA5 homeobox A5, EDU 5-ethynyl-2′-deoxyuridine, NC negative control, AML acute myeloid leukemia, Bcl-2 B-cell lymphoma 2, Bax Bcl-2 associated X, MCP-1 monocyte chemoattractant protein 1. The results were measurement data. Comparisons between two groups were conducted by means of independent sample t-test. The experiment was independently repeated three times

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us