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Fig. 6 | Cancer Cell International

Fig. 6

From: Large tumor suppressor kinase 2 overexpression attenuates 5-FU-resistance in colorectal cancer via activating the JNK-MIEF1-mitochondrial division pathway

Fig. 6

LATS2 overexpression affects 5-FU-triggered mitochondrial fission via MIEF1. a, b Proteins were isolated from SW480 cells and then the expression of MIEF1 was determined via western blotting. siRNA against MIEF1 was transfected into 5-FU/LATS2-treated SW480 cells. c, d Immunofluorescence assay for cyt-c. The expression of nuclear cyt-c was determined. eg Cellular anti-oxidants were determined via ELISA. siRNA against MIEF1 was transfected into 5-FU/LATS2-treated SW480 cells. h Caspase-9 activity was evaluated via ELISA. siRNA against MIEF1 was transfected into 5-FU/LATS2-treated SW480 cells. i The content of LDH in the medium was evaluated via ELISA. SW480 cells were treated with 5-FU and/or transfected with LATS2 adenovirus. siRNA against MIEF1 was transfected into 5-FU/LATS2-treated SW480 cells. *p < 0.05 vs. control (ctrl) group; #p < 0.05 vs. 5-FU + ad-ctrl group; @p < 0.05 vs.5-FU + ad-LATS2 + si-ctrl group; ad-ctrl: control null adenovirus vectors; ad-LATS2: LATS2 adenovirus vectors; si-MIEF1: siRNA against MIEF1

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