Fig. 3

Overexpression of USP6NL promoted CRC cell growth via activating Wnt/β-catenin pathway. a Successful establishment of USP6NL overexpression within SW480 cells, assessed by RT-PCR and western blot. USP6NL overexpression transfected SW480 cells were treated with 10 μmol/L XAV939 (Wnt/β-catenin pathway inhibitor), and then b cell proliferation. c SW480 cell proportion in G0–G1, G2 and S phases, and d protein expression of β-catenin, Cyclin D1, C-myc and P27, were assessed using CCK-8 method, flow cytometry, and western blot, respectively. ^##P < 0.01 vs. vector; ^&&P < 0.01 vs. vector + XAV939