Fig. 3

SNORA7B knockdown inhibits tumorigenicity and promotes apoptosis in BC cells. a qRT-PCR analysis of SNORA7B expression of MDA-MB-231 and BT-549 cell lines treated with specific ASOs against SNORA7B. b, c Colony formation assays were performed to assess the cell growth after SNORA7B knockdown. d, e CCK8 assays were used to assess the proliferative capacity of BC cells transfected with anti-SNORA7B-ASOs. f, g Effect of SNORA7B downregulation on migration was examined by migration assays. h, i Invasion ability of BC cells was accessed by invasion assays. j Annexin V/PI (propidium iodide) staining was performed in MDA-MB-231 and BT-549 cell lines with suppression of SNORA7B. k Percentage of apoptosis (early apoptotic cells and late apoptotic cells) in SNORA7B suppressed BC cells. (These experiments were repeated independently for three times. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 (Student’s t test).)