Fig. 3

Apatinib does not lead to significant apoptosis in PC9GR cells. a The proportions of PC9GR and PC9 cell apoptosis after treatment with apatinib for 48 h, determined by Annexin V-FITC and PI staining. No significant differences were seen at lower concentration ( < 8 μM) across cell lines at the same concentration or in the same cell lines between different concentration although slightly increased late apoptosis was observed in PC9GR cells at 16 μM. b The mitochondrial membrane potential of PC9GR and PC9 cells treated with apatinib for 24 h, measured by flow cytometry with JC-1 staining. No obvious differences were observed between PC9 and PC9GR cells