Fig. 3

SC66 suppressed cell growth by inhibiting AKT, activating GSK-3β/Bax and diminishing Bcl-xL. a P-AKT, AKT, P-GSK-3β and GSK-3β expression were analyzed by western blotting in HCT-116 at 30 min after 2 μg/ml SC66 treatment. b Puma, Bcl-2, Bcl-xL and Mcl-1 were detected in HCT-116 cells after SC66 for 24 h. c AKT, GSK-3β and Bcl-xL, expressions were analyzed by western blotting in RKO and DLD1 after 2 μg/ml SC66 treatment for 24 h. The levels of P-Akt (S473), Akt P-GSK-3β, GSK-3β and Bcl-xL were analyzed by Western blotting. Similar results were obtained from three independent experiments. d HCT-116 WT were transfected with either empty vector or a constitutively-active Akt expression constructs for 48 h, then 2 μg/ml SC66 was added into the culture medium and kept for 12 h, finally cells were harvested to detect the results. The levels of P-Akt (S473), Akt P-GSK-3β, GSK-3β and Bcl-xL were analyzed by Western blotting. Similar results were obtained from three independent experiments