Fig. 5
From: Targeting Akt by SC66 triggers GSK-3β mediated apoptosis in colon cancer therapy
SC66 induced AKT/GSK-3β/Bax-dependent apoptosis. a Colony formation was analyzed by crystal violet staining in HCT-116 WT, GSK-3β knockdown or Bax−/− after 24 h of 2 μg/ml SC66 treatment. b Cell viability was analyzed using Cell Counting Kit-8 after SC66 treatment in HCT-116 WT, GSK-3β knockdown or Bax−/−. Data represent the mean ± SEM of four independent experiments. c Hoechst 33242 morphological examination of apoptosis in HCT-116 WT, GSK-3β knockdown or Bax−/− cells were treated with 2 μg/ml SC66 and incubated for 24 h, then stained with Hoechst 33342. d Cell apoptosis was analyzed by fluorescence-activated cell sorting (FACS) analysis after in HCT-116 WT, GSK-3β knockdown or Bax−/− were treated with 2 μg/ml SC66 for 24 h. The percentage of apoptotic cells were calculated from FACS analysis. Data represent the mean ± SD of three independent experiments. ***P < 0.001 vs untreated control cells