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Fig. 3 | Cancer Cell International

Fig. 3

From: Identification of the xenograft and its ascendant sphere-forming cell line as belonging to EBV-induced lymphoma, and characterization of the status of sphere-forming cells

Fig. 3

Evaluation of the number of clonotypic B cells with the rearranged VDJ locus in the total number of cells grafted. a An image of the sphere in transmitted light (1) and the sphere containing the TAMRA+ cell (2) obtained using a laser scanning microscope. b PCR with DNA isolated from the same cells as those used to produce the graft, using JH/FR3c primers. The arrow indicates the 500 bp fragment of the differentiated B clone. c Comparison of several clones sequences (3 out of 15) with each other and also with the sequence of the B clone from the graft DNA (500, shown in red). The V, D, and J segments are shown in colors corresponding to those in Fig. 2b. The arrow indicates the primer sequence. The brackets indicate the site for cloning into the plasmid PUC19 (at the EcoRV site). The sequences are identical. Individual substitutions are shown in violet. d Quantification of the B clone-specific DNA content in the total DNA isolated from reported cell line. The calibration curve was obtained by titrating purified DNA of the B clone (500 bp) and JH/FR3c primers. The dot denotes the number of VDJ copies in studied DNA, which is calculated using the calibration curve slope

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Cancer Cell International

ISSN: 1475-2867

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