Fig. 4
From: Knockdown of PKM2 enhances radiosensitivity of cervical cancer cells
Knockdown of PKM2 induced G2/M-phase arrest and apoptosis. a Cell cycle distribution was measured through flow cytometry. Graphic representation of flow cytometry data showing percentage of cells in G1, S, and G2/M phase. b After irradiation (IR), cells were incubated for 48 h and measured through Annexin V-APC/7-AAD staining and flow cytometry. Knockdown of PKM2 significantly promoted cell apoptosis after radiation treatment. c Graphic representation of flow cytometry data showing percentage of cells in G1, S, and G2/M phases. *P < 0.05, ***P < 0.001. d Statistical images of cell apoptosis (*P < 0.05, **P < 0.01). e The proteins p-ATM, p-BRCA1, p-Chk1, p-Chk2, Cyclin B1, p-P53, and γ-H2AX were detected through Western blotting. GAPDH was used as an internal reference. Data represent three independent experiments. P phosphate