Fig. 2

Effect of different relative expression of JAG2 on the migration and invasion ability of colorectal cancer cell line HT29 and its correlation with the EMT pathway. a JAG2 siRNA inhibited the migration and invasion of human colorectal cancer cells. Migration and invasion assay of HCT116 cells following transfection with NC siRNA or JAG2 siRNA using the transwell apparatus. Microscopic analysis of migrated or invaded cells stained with DAPI (left). Relative cell migration or invasion was determined by the number of the DAPI-stained cells (right). Cell migration or invasion is expressed as a percentage of that observed in control. b JAG2 overexpression promoted the migration and invasion of human colorectal cancer cells. Migration and invasion assay of HT29 cells following transfection with pCMV-control or pCMV-JAG2 vectors. c No significant changes of EMT markers were detected with the altered JAG2 expression in human colorectal cancer cells. JAG2 and EMT markers (E-cadherin and vimentin) protein levels were examined by Western Blot analyses in HCT116 and HT29 cells treated with JAG2 siRNA or PCMV-JAG2 vectors. β-actin served as an internal control. Data are the mean ± SD. of three independent experiments. *P < 0.05 versus control