Fig. 8

Down-regulated of TDRG1 expression inhibited CC cell growth through reducing MAPK1 level in vivo. Mice were inoculated with Hela cell line stably transfected with shRNA TDRG1 or shNC plasmid. a Tumors collected from mice were shown. The effect of TDRG1 silencing on tumor volume curve and tumor weight was analyzed. b The effect of decreased TDRG1 on the MAPK1 protein level in vivo was measured by western blot. c The role of interference of TDRG1 on the miR-326 expression level in vivo was measured by qRT-PCR. d The proliferation marker Ki67 and TDRG1 expression level in vivo were detected by IHC assay. Representative photos were shown. Bar = 200 μm. Data were expressed as mean ± SD. ***P < 0.001