Fig. 6

DXO-mediated NPL4 suppresses T24 cell proliferation by regulating cyclin D1 expression. a CoIP detected the interaction between DXO and NPL4 by using CDC48 antibody in NPL4-transfected T24 cell after treatment with or without MG-132. b qRT-PCR detected the DXO mRNA expression in T24 cells treatment as above. c T24 cells were transfected with siRNAs. DXO and cyclin D1 protein levels were detected by western blotting. Right panel shows densitometric analysis. *P < 0.05, ***P < 0.001 vs. corresponding controls. d T24 cells were prepared as above, and colony formation assays were used to exam cell viability. Up panel shows colony number analysis. *P < 0.05 vs. corresponding control