Fig. 2

Enforced expression of TUBA4B impedes GC aggressive phenotype both in vitro and in vivo. a qRT-PCR analysis of TUBA4B expression in TUBA4B-overexpressing MGC-803 and HGC-27 cells. b–d CCK-8 and colony formation assays for testing the proliferative abilities of MGC-803 and HGC-27 cells with or without TUBA4B overexpression. e Transwell assay using chamber coated with matrigel for assessing the invasive capabilities of MGC-803 and HGC-27 cells with or without TUBA4B overexpression. f Annexin V and 7-AAD double staining for detecting the apoptotic rate in MGC-803 and HGC-27 cells with or without TUBA4B overexpression. g Representative image showing subcutaneous tumors of nude mice in the indicated two groups, as well as the statistical results of the volume and weight of tumors. h, i Representative images showing lung metastasis in the indicated two groups monitored by IVIS Lumina II system and H&E staining. *p < 0.05, **p < 0.01, ***p < 0.001