Fig. 7

Knockdown of DKK1 promoted the mRNA and protein expression of MMP7 in MCF-7 and MDA-MB-231 cells. Real time PCR analysis suggested overexpression of DKK1 enhanced the mRNA expression of MMP7 in MCF-7 (a) and MDA-MB-231 (b) cells. Results are presented as mean ± SEM, *p < 0.05, **p < 0.01 compared to the empty vector group, ^#p < 0.05, ^##p < 0.01 compared to the naive group, n = 5 per group, one-way ANOVA. Naïve: untreated group, Empty: empty vector group, shDKK1: DKK1 knockdown group. Western blot assay showed overexpression of DKK1 inhibited the protein expression of MMP7 in MCF-7 (c) and MDA-MB-231 (d) cells. Results are presented as mean ± SEM, *p < 0.05 compared to the empty vector group, ^#p < 0.05 compared to the naive group, n = 5 per group, one-way ANOVA. Naïve: untreated group, Empty: empty vector group, shDKK1: DKK1 knockdown group