Fig. 3

Reversine induces apoptosis by modulating capsase-3 and bax/bcl-2 in breast cancer cell lines. The levels of apoptosis-related proteins were tested with Western blot (left panel) analysis at the indicated concentrations of reversine for 48 h in MDA-mB-231 (a), MCF7 (b) and 4T1 (c) cell lines, and the relative expression of each proteins (left panels) was listed as bar grafts (n = 2) compared with the control, *p < 0.05, **p < 0.01, ***p < 0.001 (one-way ANOVA). d Heatmap of the three genes of CASP3, BAX, and BCL2 expressed in normal and breast cancer tumor tissues; data were acquired from UALCAN (see the materials and method for details). e Transcript expression levels of CASP, BAX, and BCL2 in BRCA based on breast cancer subclasses, indicating the over-expressed CASP3 and BAX genes and under-expressed BCL2 gene in the tumor tissues; data were acquired from UALCAN by analyzing the TCGA samples