Fig. 6

YAP regulated the activation of the mTOR pathway in HCC cells. a–f The protein levels of mTOR, p-mTOR, S6, p-S6, 4E-BP1 and p-4E-BP1 were analysed in HCC cell lines (BEL/FU, SK-Hep1, BEL-7402, and HCC-LM3) with YAP overexpression or inhibition by shRNA or the antagonist verteporfin. The inhibition of the mTOR pathway induced by YAP knockdown was partially reversed by NAC (0.75 mM) treatment. g, h The expression of YAP, p-mTOR and p-S6 was examined in the HCC tissue microarray (n = 120) by IHC (scale bar: 50 µm/25 µm). YAP levels were positively correlated with the expression of p-mTOR and p-S6 based on the IRS