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Fig. 1 | Cancer Cell International

Fig. 1

From: Interfering cellular lactate homeostasis overcomes Taxol resistance of breast cancer cells through the microRNA-124-mediated lactate transporter (MCT1) inhibition

Fig. 1

Lactate homeostasis is positively correlated with Taxol resistance of breast cancer cells. a BT474, SKBR3 and MCf7 cells were treated with indicated concentrations for 48 h, the protein levels and (bd) mRNA levels of LHDA and MCT1 were detected by Western blot and qRT-PCR, respectively. eg Lactate productions of BT474, SKBR3 and MCF7 cells were measured under indicated Taxol treatments. h Cell viability assays were performed in MCF7 parental and Taxol resistant cells under 0, 5, 10, 20, 40, 80, 160 and 320 nM Taxol treatments for 48 h. i Protein and (j, k) mRNA expressions of MCT1 and LDHA were detected in MCF7 parental and Taxol resistant cells by Western blot and qRT-PCR, respectively. β-actin is a loading control. l Glucose consumption and (M) lactate production were measured in MCF7 parental and Taxol resistant cells. *p < 0.05; **p < 0.01; ***p < 0.001

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