Fig. 4

PDCD4 inhibited the proliferation and migration of HCC cells. a Overexpression efficacy of PDCD4 in Huh7 and HepG2 cells by western blot. b, c PDCD4 overexpression inhibited the proliferation and migration of Huh7 and HepG2 cells by CCK8 and transwell assays. d Knockdown efficacy of PDCD4 in Huh7 and HepG2 cells by western blot analysis. e, f Knockdown of PDCD4 enhanced the proliferation and migration of Huh7 and HepG2 cells by CCK8 and transwell assays. g Western blot analysis of cell cycle regulatory proteins in different HCC cells after transfection with PDCD4 and siPDCD4. h The cell cycle distribution of HepG2 and Huh7 cells after PDCD4 upregulation or downregulation was analyzed by flow cytometry. i Gray value of cleaved caspase 3, 8, 9 and PARP protein bands in HepG2 cells after transfection with PDCD4 and siPDCD4 examined by Western blot analysis. Error bars represent the mean ± SD from three independent experiments. *p < 0.05