Fig. 5

Effects of CDDP-induced ROS levels after p53 overexpression, dominant negative Akt overexpression and AAkt overexpression in chemoresistant (H1299) and chemosensitive (A549) NSCLC cells. a H1299 cells were infected with p53 adenovirus or adeno-GFP as control (MOI = 0, 0.25, 0.5 and 1; 5 h) and treated with CDDP (10 μM, 24 h). H1299 cells were stained with dichlorofluorescin diacetate (DCFDA) and ROS level was measured by flow cytometry. X axis represents DCFDA intensity and Y axis denotes cell count. Mean FL1 is equivalent of the area under the peak, representing fluorescence intensity. c H1299 cells were infected with DN-Akt adenovirus or adeno-GFP as control (MOI = 0, 30 and 60; 5 h) and treated with CDDP (10 μM, 24 h) and ROS was measured by flow cytometer. e A549 cells were infected with AAkt adenovirus or adeno-GFP as control (MOI = 0, 30 and 60; 5 h) and treated with CDDP (10 μM, 24 h). ROS was measured by flow cytometer. b, d, f Quantification and Statistical analysis of ROS production were conducted. Data, presented as mean ± SEM (n = 3), were analyzed by two-way ANOVA and Bonferroni post hoc test (*P < 0.05, **P < 0.01, ***P < 0.001; CTL vs. CDDP)