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Fig. 2 | Cancer Cell International

Fig. 2

From: Human colorectal cancer-derived carcinoma associated fibroblasts promote CD44-mediated adhesion of colorectal cancer cells to endothelial cells by secretion of HGF

Fig. 2

Human colorectal cancer-derived CAFs promoted CD44-dependent adhesion and migration in vitro. a The adhered SW48 were counted under fluorescence microscopy (bottom) and quantified by flow cytometry (top) (n = 6), adhered LOVO and SW48 was quantified in bottom. *P < 0.05, ***P < 0.001, ****P < 0.0001. b Expression of CD44 after transfection of CD44 shRNA was analyzed by western-blot and flow cytometry. c Transwell invasion (bottom) and migration assay (top) of SW48 cells and LOVO exposed to CC-CAFs-CM with or without transfection of CD44 shRNA (magnification, ×200). And migrated cells was quantified (n = 9), error bars represent mean ± s.d; **P < 0.01; ***P < 0.001; ****P<0.0001; by one-way analysis of variance (ANOVA)

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