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Fig. 1 | Cancer Cell International

Fig. 1

From: miR-145 supports cancer cell survival and shows association with DDR genes, methylation pattern, and epithelial to mesenchymal transition

Fig. 1

SMAD3, DR5 and BRCA2 as the novel targets of miR-145: a Predicted 58 common targets for miR-145 in all the three tools (PicTaR, mirDB and miRanda), which included SMAD3, DR5 and BRCA2 with highest score; b binding sites of miR-145 identified at the 3′UTR region of the three targets, SMAD3, DR5 and BRCA2; c Luc gene activity showing a significant decrease when miR-145 was over-expressed in cells expressing Luc with 3′UTR of SMAD3 or DR5 or BRCA2, in MCF7 and HepG2 cells; d real-time PCR using Sybergreen, showing a significant decrease and increase in fold change of mRNA expression of identified targets (SMAD3, DR5 and BRCA2) in MCF7 cells under miR-145 and anti-miR-145 transfected conditions, respectively. e Western blotting of SMAD3 in presence and absence of miR-145 in MDAMB231 cell line

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