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Fig. 5 | Cancer Cell International

Fig. 5

From: Interaction between 17β-estradiol, prolactin and human papillomavirus induce E6/E7 transcript and modulate the expression and localization of hormonal receptors

Fig. 5

Expression and location of PRLR in HaCaT cells transduced with the E6 or E7 oncogenes from HPV-16 and 18. a Relative expression of PRLR mRNA was measured by qPCR, the fold change was calculated by 2−ΔΔcq method using ACTB as control. b, c PRLR protein expression was determined by western blot, densitometric analysis was performed using β-actin as control. d PRLR immunofluorescence was done using a secondary antibody conjugated with Alexa Fluor 488 (Green) and DAPI stain (blue), the images are shown in magnification 40×. e PRLR location was observed in cytoplasm and nuclei in immunofluorescence images (*p < 0.05; **p < 0.01; ***p < 0.001)

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Cancer Cell International

ISSN: 1475-2867

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