Fig. 5

The effects of EEL treatment on the activity of PI3K/Akt pathway. Bel-7402 and Huh-7 cells in control, EEL1, EEL2 and EEL3 groups were respectively treated with 0, 25, 50 and 75 mg/ml EEL for 24 h. a, b Phosphorylation levels of PI3K and Akt in Bel-7402 were detected using Western blotting assay. c, d Phosphorylation levels of PI3K and Akt in Huh-7 were detected using Western blotting assay. Data were shown as mean ± S.D. for three independent experiments. ^*P < 0.05, ^**P < 0.01 versus control